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wga cf 488a  (Biotium)


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    Structured Review

    Biotium wga cf 488a
    Wga Cf 488a, supplied by Biotium, used in various techniques. Bioz Stars score: 96/100, based on 472 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/wga+cf+488a/Wheat+Germ+Agglutinin/bio_rxiv__64898__2026__04__24__718944-101-14-15
    Average 96 stars, based on 472 article reviews
    wga cf 488a - by Bioz Stars, 2026-11
    96/100 stars

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    Related Articles

    Membrane:

    Article Title: Extracellular vimentin is expressed at the rear of activated macrophage-like cells: Potential role in enhancement of migration and phagocytosis
    Article Snippet: .. The cell membrane was stained using a WGA CF 488A (Biotium) or Alexa Fluor 549 conjugated wheatgerm agglutinin (WGA) conjugate (W11262, Invitrogen). ..

    Article Title: Extracellular vimentin is expressed at the rear of activated macrophage-like cells: Potential role in enhancement of migration and phagocytosis.
    Article Snippet: .. The cell membrane was stained using a WGA CF 488A (Biotium) or Alexa Fluor 549 conjugated wheatgerm agglutinin (WGA) conjugate (W11262, Invitrogen). ..

    Staining:

    Article Title: Extracellular vimentin is expressed at the rear of activated macrophage-like cells: Potential role in enhancement of migration and phagocytosis
    Article Snippet: .. The cell membrane was stained using a WGA CF 488A (Biotium) or Alexa Fluor 549 conjugated wheatgerm agglutinin (WGA) conjugate (W11262, Invitrogen). ..

    Article Title: Extracellular vimentin is expressed at the rear of activated macrophage-like cells: Potential role in enhancement of migration and phagocytosis.
    Article Snippet: .. The cell membrane was stained using a WGA CF 488A (Biotium) or Alexa Fluor 549 conjugated wheatgerm agglutinin (WGA) conjugate (W11262, Invitrogen). ..

    Article Title: The role of pneumococcal extracellular vesicles on the pathophysiology of the kidney disease Hemolytic Uremic Syndrome
    Article Snippet: After isolating Sp-EVs as described above, vesicles were incubated for 30 minutes at room temperature with 4’,6-diamidino-2-phenylindole, dihydrochloride (DAPI, Biotium ® ) or DiD (Vibrant TM Cell-Labeling solutions, Molecular Probes), washed with PBS, and resuspended in growth medium. .. HUVECs were seeded on 18 mm Ø coverslips in the wells of 24-well plates (7 × 10 4 cells/well), and when confluency was reached, cells were washed with DPBS and processed for either immunoblotting staining or were directly stained with WGA CF ® 488A (Biotium ® ) for 30 minutes at 37°C in 5% CO 2 . .. For immunoblot staining, HUVECs were first incubated with pre-stained vesicles, blocked (PBS with 0.5% [v/v] Tween20, 0.5% [w/v] Bovine Serum Albumin and 4% [w/v] milk), and further incubated for 1 h at room temperature with FITC-labeled anti-PECAM-1 antibody (Platelet Endothelial Cell Adhesion Molecule-1, Abcam) diluted 1:1000 in blocking buffer before being washed twice with PBS-T. Endothelial cells were incubated with pre-stained vesicles for 2 h (adhesion) or 4 h (internalization) at 37°C in 5% CO 2 , and then fixed with 4% (v/v) paraformaldehyde (PFA) for 20 minutes at 4°C.

    Whole Genome Amplification:

    Article Title: Extracellular vimentin is expressed at the rear of activated macrophage-like cells: Potential role in enhancement of migration and phagocytosis
    Article Snippet: .. The cell membrane was stained using a WGA CF 488A (Biotium) or Alexa Fluor 549 conjugated wheatgerm agglutinin (WGA) conjugate (W11262, Invitrogen). ..

    Article Title: Extracellular vimentin is expressed at the rear of activated macrophage-like cells: Potential role in enhancement of migration and phagocytosis.
    Article Snippet: .. The cell membrane was stained using a WGA CF 488A (Biotium) or Alexa Fluor 549 conjugated wheatgerm agglutinin (WGA) conjugate (W11262, Invitrogen). ..

    Article Title: The aging modulator miR-29 is essential for adult cardiomyocyte function
    Article Snippet: .. Then, tissue sections were subsequently blocked with 5% BSA in TBS-T, and incubated with WGA-CF®488A (Biotium, #29022) for 2 h. Afterwards, green channel fluorescence of the samples was imaged in a Zeiss Axio Observer microscope under a Plan-Neofluar 20x/0.50 Ph 2 objective. ..

    Article Title: The role of pneumococcal extracellular vesicles on the pathophysiology of the kidney disease Hemolytic Uremic Syndrome
    Article Snippet: After isolating Sp-EVs as described above, vesicles were incubated for 30 minutes at room temperature with 4’,6-diamidino-2-phenylindole, dihydrochloride (DAPI, Biotium ® ) or DiD (Vibrant TM Cell-Labeling solutions, Molecular Probes), washed with PBS, and resuspended in growth medium. .. HUVECs were seeded on 18 mm Ø coverslips in the wells of 24-well plates (7 × 10 4 cells/well), and when confluency was reached, cells were washed with DPBS and processed for either immunoblotting staining or were directly stained with WGA CF ® 488A (Biotium ® ) for 30 minutes at 37°C in 5% CO 2 . .. For immunoblot staining, HUVECs were first incubated with pre-stained vesicles, blocked (PBS with 0.5% [v/v] Tween20, 0.5% [w/v] Bovine Serum Albumin and 4% [w/v] milk), and further incubated for 1 h at room temperature with FITC-labeled anti-PECAM-1 antibody (Platelet Endothelial Cell Adhesion Molecule-1, Abcam) diluted 1:1000 in blocking buffer before being washed twice with PBS-T. Endothelial cells were incubated with pre-stained vesicles for 2 h (adhesion) or 4 h (internalization) at 37°C in 5% CO 2 , and then fixed with 4% (v/v) paraformaldehyde (PFA) for 20 minutes at 4°C.

    Incubation:

    Article Title: The aging modulator miR-29 is essential for adult cardiomyocyte function
    Article Snippet: .. Then, tissue sections were subsequently blocked with 5% BSA in TBS-T, and incubated with WGA-CF®488A (Biotium, #29022) for 2 h. Afterwards, green channel fluorescence of the samples was imaged in a Zeiss Axio Observer microscope under a Plan-Neofluar 20x/0.50 Ph 2 objective. ..

    Fluorescence:

    Article Title: The aging modulator miR-29 is essential for adult cardiomyocyte function
    Article Snippet: .. Then, tissue sections were subsequently blocked with 5% BSA in TBS-T, and incubated with WGA-CF®488A (Biotium, #29022) for 2 h. Afterwards, green channel fluorescence of the samples was imaged in a Zeiss Axio Observer microscope under a Plan-Neofluar 20x/0.50 Ph 2 objective. ..

    Microscopy:

    Article Title: The aging modulator miR-29 is essential for adult cardiomyocyte function
    Article Snippet: .. Then, tissue sections were subsequently blocked with 5% BSA in TBS-T, and incubated with WGA-CF®488A (Biotium, #29022) for 2 h. Afterwards, green channel fluorescence of the samples was imaged in a Zeiss Axio Observer microscope under a Plan-Neofluar 20x/0.50 Ph 2 objective. ..

    Western Blot:

    Article Title: The role of pneumococcal extracellular vesicles on the pathophysiology of the kidney disease Hemolytic Uremic Syndrome
    Article Snippet: After isolating Sp-EVs as described above, vesicles were incubated for 30 minutes at room temperature with 4’,6-diamidino-2-phenylindole, dihydrochloride (DAPI, Biotium ® ) or DiD (Vibrant TM Cell-Labeling solutions, Molecular Probes), washed with PBS, and resuspended in growth medium. .. HUVECs were seeded on 18 mm Ø coverslips in the wells of 24-well plates (7 × 10 4 cells/well), and when confluency was reached, cells were washed with DPBS and processed for either immunoblotting staining or were directly stained with WGA CF ® 488A (Biotium ® ) for 30 minutes at 37°C in 5% CO 2 . .. For immunoblot staining, HUVECs were first incubated with pre-stained vesicles, blocked (PBS with 0.5% [v/v] Tween20, 0.5% [w/v] Bovine Serum Albumin and 4% [w/v] milk), and further incubated for 1 h at room temperature with FITC-labeled anti-PECAM-1 antibody (Platelet Endothelial Cell Adhesion Molecule-1, Abcam) diluted 1:1000 in blocking buffer before being washed twice with PBS-T. Endothelial cells were incubated with pre-stained vesicles for 2 h (adhesion) or 4 h (internalization) at 37°C in 5% CO 2 , and then fixed with 4% (v/v) paraformaldehyde (PFA) for 20 minutes at 4°C.



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